Catalog Number - 68322-10P

The 68322‑10P Cogent RP PFP™ HPLC Column is designed for enhanced selectivity of halogenated, aromatic, and polar compounds, delivering improved resolution where traditional C18 phases may not fully separate structurally similar analytes.

This stationary phase utilizes a pentafluorophenyl (PFP, “F5”) ligand bonded via a four‑carbon linker to high‑purity silica with full double end‑capping, enabling multiple interaction mechanisms including π‑π interactions, dipole interactions, and hydrogen bonding. These combined interactions provide greater selectivity control and improved separation performance.

The 100 Å pore size offers broad versatility across small to moderately sized molecules, making this column a strong choice for routine analytical workflows and method development.

100 mm Length – Increased Resolution for Analytical Workflows

The 100 mm length provides an optimal balance of resolution and run time, improving separation compared to 50 mm columns while maintaining efficiency.

• Better resolution than short screening columns

• Improved separation of closely related compounds

• Increased peak capacity for complex samples

✅ Use when greater resolving power is required without extending to longer columns

Flow Rate & System Requirements (4.6 mm ID, 3 µm)

⚠️ Compatible with standard HPLC systems, with moderate backpressure

• Typical flow rate range: ~0.8–1.5 mL/min

• Higher efficiency than 5 µm columns

• Increased backpressure vs larger particles

• Compatible with standard analytical detectors

✅ Sample preparation requirement: filter samples using a 0.2 µm syringe filter prior to injection

Why Choose PFP (F5) for Selective Separations

Enhanced Retention for Halogenated Compounds

• Strong interaction with fluorinated and halogenated analytes

• Improved selectivity vs C18

Multiple Interaction Mechanisms

• π‑π interactions for aromatic compounds

• Dipole and hydrogen bonding interactions

• Greater flexibility for method development

Versatile 100 Å Pore Structure

• Suitable for a wide range of analytes

• Balanced performance across applications

Cogent RP PFP™ Advantages

Fully End‑Capped Surface

• Minimizes silanol activity

• Improves peak symmetry

High Efficiency (3 µm Particles)

• More efficient than 5 µm columns

• Sharper peaks and improved resolution

High Purity Silica

• Consistent chromatographic performance

• Reliable reproducibility across runs

Typical Applications

• Halogenated compound analysis

• Aromatic compound separations

• Pharmaceutical QA/QC workflows

• Method development and optimization

• USP L43 method applications

Important Usage Notes

• Packed and shipped in reversed‑phase solvents

• Designed for standard analytical HPLC systems (4.6 mm ID)

• Higher backpressure than larger particle sizes

• Use 0.2 µm filtration to maintain column performance